During the baseline survey, 50 ml non-fasting blood sample (with time of last meal recorded) from each participant was collected into SST, EDTA, ACD, PST, Oxalate and RNA Tempus vacutainer. The samples were then kept in a portable, insulated cool box with ice packs (to maintain their temperature at 0-4 °C) for up to a few hours before being taken to the local study laboratory for immediate processing.
Samples were processed analysed and then aliquoted for long term storage.
SST: for measuring serum blood chemistry. Biochemistry profile includes : cholesterol, HS C-reactive protein, HDL, GGT, HbA1c, glucose, Apo-lipoprotein A1, Apo-lipoprotein b, urea, Creatinine, C-peptide.
Sodium citrate: Coagulation analysis (PT, fibrinogen)
EDTA: haematology analysis and HbA1c. Haematology profile includes RBC, WBC, Haemoglobin, haematocrit (HCT) ,Mean cell volume(MCV), Mean cell haemoglobin (MCH), Mean cell haemoglobin concentration (MCHC), platelets, DLC.
PST: Plasma for metabolomics.
Flouride oxalate: Measures blood glucose.
ACD: Cell cryopreservation for future use.
RNA Tempus: For future RNA extraction